Brdu birthdating protocol


We have also developed the Click-i T® Ed U Microplate Assay that employs the nucleoside analog Ed U (5-ethynyl-2’-deoxyuridine) and a detection method that is not antibody-based, so it does not require DNA denaturation.The advantage of these incorporation assays is that they are direct measures of proliferation.When a scientist injects Brd U into the bloodstream of a test animal, the chemical becomes available to all cells – most importantly, those which are proliferating.

Brd U is commonly used in the detection of proliferating cells in living tissues.

(See a list of the products featured in this article.) Cell proliferation assays provide a critical piece of the puzzle when evaluating cell health, genotoxicity, and the efficacy of anti-cancer drugs.

Proliferation, however, is rarely assayed in isolation; other cell function probes are often used in concert with proliferation assays to provide a more informative picture of the state of the cell.

Cell proliferation can be measured with the thymidine analog Brd U (5-bromo-2’-deoxyuridine) following its incorporation into newly synthesized DNA and its subsequent detection with an anti-Brd U antibody.

Bromodeoxyuridine, variously abbreviated as Brd U, Bud R, and Brd Urd, is a halogenated thymidine analog that is permanently integrated into the DNA of dividing cells during DNA synthesis in S phase. (2008) Identification of Newborn Cells by Brd U Labeling and Immunocytochemistry In Vivo.

Since the two chemicals are analogous, some spots of the genetic code that call for Thymidine will instead receive Brd U.

You must have an account to comment. Please register or login here!